Restriction site associated DNA markers (RAD markers or RAD tags) are used in association with high-throughput sequencing methods for genotyping in both model and non-model organisms.

RAD tags are produced by using restriction enzymes to cut an organism DNA and to perform a genome complexity reduction. In a RAD-seq experiment, only those fragments associated with restriction sites are sequenced. The distribution of sequenced fragments along the genome can be adjusted by carefully choosing the restriction enzymes.

RAD tag course in Roscoff, March 2015

The local organizers of the course are Thomas Broquet and Didier Jollivet. The course will be taught by Matthieu Bruneaux and Gildas Le Corguillé.

All the materials are available from GitHub.

Before the course

Please fill the pre-assessment form that can be found here and send it back to Matthieu Bruneaux (matthieu.d.bruneaux@jyu.fi).

Schedule

The practicals will take place on 10th, 11th and 12th of March at the Station Biologique de Roscoff, in the computer room (IGM).

  • 2015-03-10 Tue (Afternoon): RAD-seq presentation (pdf), beginning of practicals
  • 2015-03-11 Wed (All day): Practicals and open questions
  • 2015-03-12 Thu (All day): Practicals and open questions, conclusion

Contact

Comments, questions and suggestions can be sent to Matthieu Bruneaux.